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A.E. Arbuzov Institute of Organic and Physical Chemistry

Subdivision of the Federal State Budgetary Institution of Science "Kazan Scientific Center of Russian Academy of Sciences"

Laboratory of highly organized structures

The Laboratory of Highly Organized Structures was created on the base of the group under the supervision of Dr. L.A. Kudryavtseva in 2000. Since 2008 the Laboratory is headed by Professor Lucia Ya. Zakharova.

 

Head of Laboratory
Full Professor, Dr. Zakharova Lucia Yarullovna
Phone number (843) 273-22-93
Room 306
Email lucia@iopc.ru

Deputy Head of the Laboratory

Senior Researcher, PhD Mirgorodskaya Alla Bencionovna
Phone number (843) 273-22-93
Room 311
Email mirgorod@iopc.ru

Members and postgraduates
Senior Researcher, PhD Zhiltsova Elena Petrovna
Senior Researcher, PhD Lukashenko Svetlana Sergeevna
Researcher, PhD Valeeva Farida Garafeevna
Researcher, PhD Gaynanova Gulnara Akhatovna
Researcher, PhD Pashirova Tatiana Nikandrovna
Researcher, PhD Ibragimova Alsu Raynurovna
Researcher, PhD Kashapov Ruslan Ravilevich
Junior Researcher, PhD Kudryashova Yuliana Radikovna
Junior Researcher, PhD Gabdrakhmanov Dinar Rashidovich
Junior Researcher, PhD Vasilieva Elmira Albertovna
Junior Researcher, PhD Yackevich Ekaterina Igorevna

 

 

History of the laboratory's development

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Laboratory of Highly Organized Structures (LHOS) is historically related to Laboratory of Organophosphorus Polymers (LOPP) under the leadership of Professor B.E. Ivanov, one of the largest and oldest laboratories of the A.E. Arbuzov Institute of Organic and Physical Chemistry (IOPC). Over 1975-1985 period, the laboratory staff comprised over 80 people and gathered leading scientific groups that carried out research on the tasks of the Presidium of the Academy of Sciences of the USSR. Eventually some of these research teams of LOPP branched out into independent laboratories headed by Professor V.S. Reznik, Professor Ya.A. Levin, Professor I.A. Nuretdinov. In 1996 the group of LHOS was separated and taken under the leadership of Dr. L.A. Kudryavtseva. At that time the main focus of the laboratory work was the study of influence of the solvents as well as micellar systems on the rate of nucleophilic substitution reactions of phosphorus acid esters. Dr. V.E. Belskiy and Dr. S.B. Fedorov played a central role in the evolution of laboratory and its creative development. Since 2008 the Laboratory is headed by Professor Lucia Ya. Zakharova.

 

Research trends

  • The main research direction of the LHOS is the development of self-assembly strategies of the design of polyfunctional nanosystems with enhanced properties (catalysis, solubilization, anticorrosion and antimicrobial activity) based on the key “bottom-up” principle of supramolecular chemistry, i.e. non-covalent self-organization of surfactants, cyclophanes, and polymers. One of the priority research lines in the LHOS concerns specifically the formation of nanosized containers and polyelectrolyte capsules for organic and biological substrates in order to identify the factors controlling substrate reactivity, preservation, concentration and biotransport. A new approach to encapsulate hydrophobic (uncharged) substrates was developed using layer-by-layer technology allowing obtaining of micro- and nanocapsules with substrate binding and release properties. Recently the laboratory activity has been concerned with the solution of fundamental problems in medical chemistry, pharmacology, and nanoengineering. From this point of view, our research interest includes the investigation of the novel supramolecular systems that sensitive to external stimuli and capable to effectively interact with cell membranes.
  • The achievement of the goals stated assumes a systematic study of self-assembly of individual amphiphilic compounds and their mixed systems, including their synthesis as a tool for progressive variation of the building block structure. This approach allows us to go from screening to the directed design of functional systems based on established regularities.
  • Biomimetic nature of supramolecular systems allows us to solve the high-priority problems of the biotechnological applications: the formation of nanocontainers, micro- and nanocapsules for storage and delivery of therapeutic agents including water-insoluble drugs, DNA, diagnostic probes.
  • Conventional direction of investigations in laboratory is fabrication of supramolecular catalytic systems using the strategy of amphiphilic compounds self-assembly for decontamination of toxic esters of phosphorus acids, acetylcholinesterase inhibitors. Collected results allows to design effective nanoreactors based on cationic surfactants, polymers, macrocycles and metal ions. It provides catalytic activity up to six and more orders of magnitude, specificity and producibility of process due to combined contribution of micellar and homogeneous catalysis, as well as immobilization of nanoreactors on polymer or inorganic template
  • Laboratory pays much attention to the collaboration with research groups working in the key areas of regional science associated with oil extraction and processing. The experience gained and knowledge of the tendency in changes of properties of surfactants in condensed state makes it possible to design the inhibitors of corrosion - bactericides for the protection of oil field equipment and viscoelastic compositions for increasing oil recovery, etc.

Important results of laboratory as a part of achievements of Arbuzov IOPC, FRC Kazan Scientific Center of RAS

  • 2013 year:

1. Novel DNA carriers based on cationic pyrimidine-containing, diterpenoid and hydroxyethylated gemini amphiphiles suitable to stringent criteria of high transfection efficiency, biocompatibility and low toxicity has been revealed and recommended.
2. Novel pH-dependent nanocontainers on the basis of nonionic surfactants and amphiphilic pyrimidinophanes has been constructed for controlled delivery of drugs. The structure of nanocontainers and binding-release of water-insoluble substrates is regulated by acid-base properties of the media and determined by capability of pyrimidinophane to reversibly change its conformation.

  • 2014 year:

The strategy of regulation of self-assembly and functional activity of calix[4]arenes and cavitands on their basis has been designed. Formulations fabricated is capable to act as drug delivery systems and allows to control surface activity, solubilization of drugs and catalytic effect in wide range by variation of the length of alkyl tails at the lower rim, solvent and counterion nature and the ratio of calix[4]resorcine-co-surfactant. Unlike conventional surfactants, the boundary length of alkyl tail of calix[4]resorcine corresponding to the switch of aggregation mode has been found.

  • 2015 year:

The method of toxicity reduction and enhancement of functional properties (solubilization ability, antimicrobial activity) of nanosystems based on cationic surfactants using its modification by hydrotropic agents has been suggested. The strategy designed allows to fabricate low-toxic nanocontainers for hydrophobic drugs and probes, as well as antimicrobial compositions comparable with commercial analogues by several characteristics.

  • 2016 year:

1. Novel biomimetic catalysts (pyrimidine-containing and phosphonium-containing amphiphiles) allowing to regulate the rate of cleavage of organophosphorous inhibitors of acetylcholinesterase (in the range from acceleration to anomalous inhibition) and exhibiting high effectiveness in low concentrations and marked substrate specificity have been suggested.
2. For the first time ever the capability of direct control of practically important properties of cationic surfactants (drug and DNA delivery, catalytic activity, antimicrobial and anticorrosion effect) by introduction of oxygen-containing moieties within head groups has been shown. High activity of morpholinium-containing and hydroxyethylated gemini surfactants is due to the low aggregation threshold, multisite mechanism of interaction and capability to penetrate through cell membranes.

  • 2017 year:

1. For the first time ever lipid nanocontainers for quaternized oximes capable to pass across blood-brain barrier have been designed. Due to the high encapsulation effectiveness of the drug (pralidoxime chloride) 15 % of acetylcholinesterase reactivation in the brain has been reached. It allows to 2-fold increase of the survival rate of rats after poisoning by lethal dose of paraoxone - wide used in the world pesticide. It is the world first successful result in the field of the therapy of the poisoning of cholinesterases by organophosphorus inhibitors using nanocontainers.
2. For the first time ever for homologous series of imidazolium-containing amphiphiles nonconventional mechanism of the influence of hydrophobicity on functional activity, differing from typical behavior of surfactant solutions (correlation between properties and the length of alkyl tails), has been exhibited. Qualitative binding of DNA decamer by all homologues, levelling of the hydrophobicity effect on solubilization power of micelles, as well as the ability of only lower homologues to integrate with lipid bilayer increasing of its permeability for drugs have been exhibited. Results obtained increases biotechnological potential of supramolecular systems.

 

Selected Publications

 

Grants Support

 

Collaborations with other Institutes and Centers

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Laboratory Equipment